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Set 1, Slide 52, Wetmount, Lightfield
Leaf Linked Set

Leaf

 

Hydrilla Verticillata Leaf Collage 40xThis hydrilla verticillata leaf has many clearly defined plant cells. The leaf has a dark stem down its middle. It's edges are serrated with sharpened little "thorns" at the tips. The surface of the leaf is similar to the onion epidermis, but the nuclei of the plant cells in the leaf are barely visible if at all.

The cells seem to be lined up into columns that all extend from one end of the leaf to its tip and which narrow as the tip is approached. Some few dark cells appear which seem to have sucked up more of the blue stain than most others.

Hydrilla Verticillata LeafHydrilla Verticillata Leaf

Surface of Leaf

This is clearly multiple layers thick. Layers are visible beneath the top, focused layer. The leaf seems to have a slighly darker row of cells near its edge. Nuclei are much less visible than with the onion root.

Image Data Description
Hydrilla Verticillata Leaf Surface 200x
200x
The darker edges of the upper layer of cells appear clear and distinct in comparison to those of the layers below. There is a "texture" to the stuff of the cells that looks like to magnification protists. I hesitate to call this stuff the cytoplasm of the cell as the texture seems too great.
Hydrilla Verticillata Leaf Surface 200x
200x
Many interesting dead organisms such as diatoms, are found on the leaf's surface.
Hydrilla Verticillata Leaf Surface 400x
400x
Here, two long thin UFO-shaped diatoms can be seen.
Hydrilla Verticillata Leaf Surface 1000x
1000x
At 1000x with oil, large organelles are visible. The top layer of cells' organelle has a definitive bubbly texture to it. I'd like to assume that this is the nucleus, but no distinct chromosomes can be seen as with the onion root tips. Their empty nature makes me wonder if they are some sort of vacuole.

Midrib

Midrib is a term for the dark line found at the middle of the leaf.

Image Data Description
Hydrilla Verticillata Leaf Midrib100x
100x
The midrib seems to have several horizontal lines on it. This may just be the accumulation of overlapping cell walls of higher-layers of cells.
Hydrilla Verticillata Leaf Midrib 400x
400x
At 400x though, the lines seem more pronounced and even a little bit "rougher".

Thorns

There are several thorns found at the tips of the leaf's serrations.

Image Data Description
Hydrilla Verticillata Leaf Thorn 200x
200x
The internal structure of the thorn is visible. It rather resembles the "quick" of a cat's claw.
Hydrilla Verticillata Leaf Thorn 1000x
1000x
At 1000x, an orange edge of the thorn is visible. This could very well be tougher material than a standard cell and more suitable for puncturing purposes.

Shaded Part

Image Data Description
Hydrilla Verticillata Leaf Dark Cells 200x
200x
Those dark bits seen in the sample image are more obviously individual cells at 200x. Probably, they just soaked up more of the stain, but they look like they don't have the electric lights of their neighbors.
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